sv40 large t antigen hek293t (ATCC)

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ATCC sv40 large t antigen hek293t
Sv40 Large T Antigen Hek293t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sv40+large+t+antigen+hek293t/293T%2F17/10__1038_slash_s41514___026___00469___4-302-22-36
Average 99 stars, based on 12205 article reviews

sv40 large t antigen hek293t - by Bioz Stars, 2026-10

99/100 stars

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Stable Transfection:

Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21 .. Human embryonic kidney cells (female) cells stably expressing the SV40 large T antigen (HEK293T) were obtained from ATCC. .. GP2293 for retroviral packaging were obtained from Clontech.

Article Title: Nuclear aging in polyglutamine-induced neurodegeneration
Article Snippet: Cells were maintained in culture using high glucose Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St. Louis, USA) supplemented with 10% fetal bovine serum (FBS, Life Technologies), 1% nonessential amino acids, 2 mM L-glutamine (Life Technologies), 1% penicillin/streptomycin (Life Technologies), 110 mg/L sodium pyruvate (Sigma-Aldrich) and 44 mM sodium bicarbonate (Sigma-Aldrich) and kept at 37oC in a 5% CO2 atmosphere incubator. .. AR TI CL E IN P RE SS Culture of human and murine cell lines Human embryonic kidney 293, stably expressing the SV40 large T antigen (HEK293T) and mouse neuroblastoma cell line (N2a) obtained from the American Type Culture Collection cell biology bank (CCL-131), were maintained in standard DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin at 37oC under a humidified atmosphere containing 5% CO2. .. Transfection of N2a cell line For the transfection of N2a cell line, 1x105 cells were plated on a 12-well cell culture multiwell plate containing poly-D lysine-coated coverslips (Sigma-Aldrich).

Expressing:

Article Title: Gender difference in pre-clinical liver-directed gene therapy with lentiviral vectors
Article Snippet: .. The human embryonic kidney cell line expressing the SV40 large T antigen (HEK293T) was obtained from ATCC (LGC Standards, Teddington, United Kingdom) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Merck Life Science, Dorset, United Kingdom) supplemented with 10% heat-inactivated fetal bovine serum (FBS; ThermoFisher Scientific, United Kingdom), 2 mM L-glutamine (Merck Life Science) and 1% non-essential amino acids (NEAAs; Merck Life Science). .. HEK293T cells adapted to culture in suspension phase were maintained in FreestyleTM 293 Expression Medium (FS; ThermoFisher Scientific) with 0.1% cholesterol lipid concentrate 250X (CLC; ThermoFisher Scientific) (FS + 0.1% CLC).

Article Title: Investigating dynamics of lentiviral vector secretion from HEK293T producer cells using a fractionated perfusion system
Article Snippet: .. Human embryonic kidney cells expressing the SV40 large T antigen (HEK293T) (ATCC: CRL-3216) were grown in DMEM/F12 medium (Gibco) supplemented with 10% FBS (Gibco) and 1% penicillin/streptomycin (Gibco) during growth stages. ..

Cell Biology:

Cell Culture:

Modification:

Transformation Assay:

Article Title: Membralin Assembles a MAN1B1–VCP Complex to Target Foreign Glycoproteins from the Endoplasmic Reticulum to Lysosomes for Degradation
Article Snippet: Commercial reagents include: Kifunensine, Eeyarestatin I, MG132, Lactacystin (Lac), Concanamycin A (ConA), 3‐Methyladenine (3‐MA), and ammonium chloride (NH 4 Cl) (Sigma–Aldrich, K1140, E1286, C2211, L6785, C9705, M9281, A9434); Bafilomycin A1 (BafA1, Santa Cruz Biotechnology, sc‐201550); mouse monoclonal anti‐Myc (CST, 2276S); rabbit polyclonal anti‐SQSTM1, mouse monoclonal anti‐FLAG and anti‐HA (Sigma‐Aldrich, P0067, F3165, H3663); mouse monoclonal anti‐ACTB/β‐actin (CST, 3700S); mouse monoclonal anti‐ERManI/MAN1B1 (Novus, NBP2‐13167); rabbit polyclonal anti‐RETREG1‐2 and anti‐Membralin (Gene Tex, GTX46621, GTX118618); rabbit polyclonal anti‐ATL3, anti‐RETREG1, anti‐CCPG1, anti‐TEX264, and anti‐RETREG2 (Proteintech, 16921‐1‐AP, 21537‐AP, 13861‐1‐AP, 25858‐1‐AP, 24650‐1‐AP); rabbit monoclonal anti‐SEC62 and rabbit polyclonal anti‐EDEM2 (Abcam, ab137022, ab181218); rabbit polyclonal anti‐RTN3 (ThermoFisher Scientific, PA5‐78316); mouse monoclonal anti‐SERPINA1/AAT (Sigma‐Aldrich, SAB4200198); rabbit polyclonal anti‐Sec61A and Sec61B (Abcam, ab183046, ab15576); rabbit polyclonal anti‐VCP (Huabio, ER30603); horseradish peroxidase (HRP)‐conjugated goat anti‐mouse IgG and anti‐rabbit IgG (Jackson ImmunoResearch Laboratories, 115‐035‐003, 111‐035‐003). .. Human embryonic kidney (HEK) 293 cell line transformed with SV40 large T antigen (HEK293T) and human cervical carcinoma cell line HeLa were purchased from American Type Culture Collection (ATCC, CRL‐3216, CRM‐CCL‐2); human hepatoma cell line Huh7 was purchased from BioBW, China (BioBW, bio‐73061); TZM‐bI cells were from NIH HIV Reagent Program. ..

Article Title: Membralin Assembles a MAN1B1-VCP Complex to Target Foreign Glycoproteins from the Endoplasmic Reticulum to Lysosomes for Degradation.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com mouse monoclonal anti-ACTB/β-actin (CST, 3700S); mouse monoclonal anti-ERManI/MAN1B1 (Novus, NBP2-13167); rabbit polyclonal antiRETREG1-2 and anti-Membralin (Gene Tex, GTX46621, GTX118618); rabbit polyclonal anti-ATL3, anti-RETREG1, anti-CCPG1, anti-TEX264, and anti-RETREG2 (Proteintech, 16921-1-AP, 21537-AP, 13861-1-AP, 25858- 1-AP, 24650-1-AP); rabbit monoclonal anti-SEC62 and rabbit polyclonal anti-EDEM2 (Abcam, ab137022, ab181218); rabbit polyclonal antiRTN3 (ThermoFisher Scientific, PA5-78316); mouse monoclonal antiSERPINA1/AAT (Sigma-Aldrich, SAB4200198); rabbit polyclonal antiSec61A and Sec61B (Abcam, ab183046, ab15576); rabbit polyclonal antiVCP (Huabio, ER30603); horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 115-035-003, 111-035-003). .. Cells: Human embryonic kidney (HEK) 293 cell line transformed with SV40 large T antigen (HEK293T) and human cervical carcinoma cell line HeLa were purchased from American Type Culture Collection (ATCC, CRL-3216, CRM-CCL-2); human hepatoma cell line Huh7 was purchased from BioBW, China (BioBW, bio-73061); TZM-bI cells were from NIH HIV Reagent Program. ..

Mutagenesis:

Article Title: Spatial chromatin accessibility sequencing resolves high-order spatial interactions of epigenomic markers
Article Snippet: .. Mouse mammary gland carcinoma cell line 4T1 and the derivative human embryo kidney cell line which expresses a mutant version of the SV40 large T antigen (HEK293T) were obtained from ATCC. .. 4T1 were grown in RPMI1640 (Gibco, 11875093) supplemented with 10% fetal bovine serum (FBS; Gibco, 10099141), and 1% penicillin–streptomycin (Gibco, 15140122).

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